A spermatozoa in order to fertilize must have intact, mature, undamaged DNA. Chromatin protects the DNA on its long dangerous journey in the female body making the spermatozoa stronger and more resistant to damage.

It is known that alterations in the sperm chromatin level above 30 % reduce the likelihood of a spontaneous pregnancy and the success of assisted fertility techniques. Poor sperm chromatin seems to be highly indicative of male sub-fertility independently of the number, motility and morphology of sperms in the semen. If there are abnormalities in the DNA this leads to non- viable embryos. Hence failure in FIVET or ICSI may be ascribed to a possible lack of maturity or to damaged spermatic DNA.


Thanks to the TUNEL Assay the treating physician has more complete information about the fertilizing ability of the patient’s spermatozoa on which to base decisions concerning further treatment.


Fertility clinics should carry out Tunel Assay as defects in the DNA make it clear why previous assisted fertility attempts have failed